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November 1, 2000AJP Heart and Circulatory PhysiologyOpen Access

Partitioning of pyruvate between oxidation and anaplerosis in swine hearts

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Key result

In normal anesthetized swine hearts, pyruvate carboxylation accounted for 4.7 +/- 0.3% of citrate formation, representing at least 3-6% of citric acid cycle flux.

Population

Normal anesthetized swine hearts

Comparison

Infusion of [U-C]lactate and/or [U-C] pyruvate… vs Different concentrations of arterial pyruvate or…

Design

Preclinical

Follow-up

1 hour

Authors

APAshish R. PanchalMedical College of WisconsinBCBlandine ComteUniversité Clermont AuvergneHHHazel HuangUniversity of Washington

Discussion

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Implication

Baseline anaplerosis quantified in normal swine; leaves open its role in human cardiac metabolism.

Structured PICO

P
Population
Normal anesthetized swine hearts
I
Intervention
Infusion of [U-(13)C(3)]lactate and/or [U-(13)C(3)] pyruvate into the left anterior descending (LAD) coronary artery (with or without 1 mM octanoate)
C
Comparator
Different concentrations of arterial pyruvate (0.2 to 1.1 mM) or addition of octanoate
O
Outcome
Flux through pyruvate carboxylation and decarboxylation (measured by mass isotopomer distribution of citrate and its oxaloacetate moiety)surrogate

In the in vivo swine heart, pyruvate carboxylation accounts for 3-6% of citric acid cycle flux and remains stable despite significant variations in pyruvate decarboxylation.

Cite This Study

Panchal et al. (2000) studied Normal anesthetized swine hearts. Infusion of [U-(13)C(3)]lactate and/or [U-(13)C(3)] pyruvate was evaluated on Flux through pyruvate carboxylation and decarboxylation (percentage of citrate formation). In normal anesthetized swine hearts, pyruvate carboxylation accounted for 4.7 +/- 0.3% of citrate formation, representing at least 3-6% of citric acid cycle flux.

synapsesocial.com/papers/6a07fdc609b3c82015379154https://doi.org/10.1152/ajpheart.2000.279.5.h2390
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Pyruvate‐enhanced phosphorylation potential and inotropism in normoxic and postischemic isolated working heart1989 · 222 citations
  2. 2Myocardial Exchanges of Glutamate, Alanine and Citrate in Controls and Patients with Coronary Artery Disease1983 · 93 citations
  3. 3Improved estimation of anaplerosis in heart using13C NMR1997 · 19 citations
  4. 4Pyruvate carboxylation as an anaplerotic mechanism in the isolated perfused rat heart1982 · 76 citations
  5. 5Changes in citric acid cycle flux and anaplerosis antedate the functional decline in isolated rat hearts utilizing acetoacetate.1991 · 172 citations