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methods, Helixer and ANNEVO, while also having the fastest runtimes overall. Compared with BRAKER3, which incorporates RNA-Seq and protein evidence, Tiberius approaches state-of-the-art accuracy in Mesangiospermae, Fungi, Bacillariophyta and Chlorophyta, while being on average 80 times faster when using a GPU. Availability and implementation: https://github.com/Gaius-Augustus/Tiberius.
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Gabriel et al. (2026) studied this question.
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