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May 16, 2026BMC Cardiovascular DisordersOpen Access

Identification of key genes related to macrophages and metabolic reprogramming in myocardial infarct based on single-cell and bulk transcriptomics data and experimental validation

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Key result

Upregulated ABCG1 and downregulated GNG11 and RPL24 are linked to macrophage metabolic reprogramming in MI.

  • P<0.05
  • n=69

Why the study?

Myocardial infarction is influenced by altered macrophage status and metabolic reprogramming, but the mechanisms of their crosstalk remain unclear.

Population

Single-cell and bulk transcriptomics data of MI from public databases

Design

Bioinformatics and transcriptomic study with experimental RT-qPCR validation

Authors

LLLiguo LangTMTing MengYQYaping Qiang

Discussion

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Overview

Should not yet change MI management; leaves open therapeutic targeting of macrophage regulators in humans.

Key Points

  • This research aims to uncover key genes related to macrophages and metabolic reprogramming in myocardial infarction.
  • Utilized single-cell and bulk transcriptomics data from public databases and literature.
  • Identified differentially expressed genes (DEGs) related to macrophages and metabolic reprogramming using bioinformatics.
  • Validated key genes through RT-qPCR and conducted drug prediction analysis.
  • Identified 6 candidate genes, including ABCG1, GNG11, and RPL24, with significant expression changes in myocardial infarction samples.
  • Key genes co-enriched in pathways related to Huntington’s disease and oxidative phosphorylation.
  • Two drugs, APT and vitamin E, targeting ABCG1 were identified.

Study Design

Type

Observational (n=69)

Multicenter

No

Structured PICO

P
Population
Single-cell and bulk transcriptomics data of myocardial infarction (MI) from public databases
I
Intervention
Bioinformatics analysis (machine learning, gene expression, ROC curve, cell communication, pseudo-temporal analyses) and RT-qPCR validation
O
Outcome
Identification of key genes associated with macrophages and metabolic reprogramming in MIsurrogate

Main Result

p-value: p=<0.05

ABCG1, GNG11, and RPL24 were identified as key regulators of macrophage function and metabolic reprogramming in myocardial infarction, offering potential therapeutic targets.

Cite This Study

Lang et al. (2026) conducted an observational in Myocardial infarction (n=69). Bioinformatics screening and RT-qPCR validation vs. Healthy controls was evaluated on Differential expression of key genes (ABCG1, GNG11, RPL24) in MI versus control (p=<0.05). ABCG1, GNG11, and RPL24 were identified as key regulators of macrophage function and metabolic reprogramming in myocardial infarction, with ABCG1 significantly upregulated and GNG11 and RPL24 downregulated.

synapsesocial.com/papers/6a080acea487c87a6a40cbedhttps://doi.org/10.1186/s12872-026-05822-9
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Identification of mitophagy-related key genes and their correlation with immune cell infiltration in acute myocardial infarction via bioinformatics analysis2025 · 2 citations
  2. 2Identification of candidate macrophage-ferroptosis crosstalk genes associated with immune infiltration in myocardial infarction: A bioinformatics analysis2026
  3. 3Identification and exploration of novel M2 macrophage-related biomarkers in the development of acute myocardial infarction2022 · 6 citations
  4. 4Identification of hub genes in myocardial infarction by bioinformatics and machine learning: insights into inflammation and immune regulation2025 · 5 citations
  5. 5Identification of marophage-related biomarkers in acute myocardial infarction (AMI) by bioinformatic analysis and clinical validation Running title: Macrophage-related biomarkers in AMI2024