Key result
Circulating miR-24-1 and miR-545 detect AMI with AUC >0.9, strongly correlating with troponin T.
Why the study?
Knowledge of the molecular mechanisms underlying AMI remains limited, and accurate biomarkers are needed because the gold standard cTnT assay requires serial testing influenced by symptom timing.
Can specific circulating miRNAs serve as diagnostic biomarkers for acute myocardial infarction?
Case-Control (n=22)
No
Can specific circulating miRNAs serve as diagnostic biomarkers for acute myocardial infarction?
Effect estimate: AUC >0.9
Eight novel circulating miRNAs were identified as potential diagnostic biomarkers for acute myocardial infarction.
May support miRNA diagnostics for AMI; hypothesis-generating and requires prospective validation before any practice change.
Background Acute myocardial infarction (AMI) is considered one of the most prominent causes of death from cardiovascular disease worldwide. Knowledge of the molecular mechanisms underlying AMI remains limited. Accurate biomarkers are needed to predict the risk of AMI and would be beneficial for managing the incidence rate. The gold standard for the diagnosis of AMI, the cardiac troponin T (cTnT) assay, requires serial testing, and the timing of measurement with respect to symptoms affects the results. As attractive candidate diagnostic biomarkers in AMI, circulating microRNAs (miRNAs) are easily detectable, generally stable and tissue specific. Methods The Gene Expression Omnibus (GEO) database was used to compare miRNA expression between AMI and control samples, and the interactions between miRNAs and mRNAs were analysed for expression and function. Furthermore, a protein-protein interaction (PPI) network was constructed. The miRNAs identified in the bioinformatic analysis were verified by RT-qPCR in an H9C2 cell line. The miRNAs in plasma samples from patients with AMI ( n = 11) and healthy controls ( n = 11) were used to construct receiver operating characteristic (ROC) curves to evaluate the clinical prognostic value of the identified miRNAs. Results We identified eight novel miRNAs as potential candidate diagnostic biomarkers for patients with AMI. In addition, the predicted target genes provide insight into the molecular mechanisms underlying AMI.
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Wang et al. (2020) conducted a case-control in Acute myocardial infarction (n=22). Circulating miRNAs (miR-24-1 and miR-545) vs. Healthy controls was evaluated on Diagnostic accuracy (AUC) for acute myocardial infarction (AUC >0.9). Circulating miR-24-1 and miR-545 demonstrated high diagnostic accuracy for acute myocardial infarction with AUC values greater than 0.9 and strongly correlated with troponin T levels.
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