May 16, 2026Journal of the American College of Cardiology
Regional myocardial blood flow, function and metabolism using phosphorus-31 nuclear magnetic resonance spectroscopy during ischemia and reperfusion in dogs
Key result
Coronary occlusion cuts transmural blood flow ~77%, acutely impairing segmental systolic function and phosphocreatine levels.
Population
Mongrel dogs (intact large animal model of regional myocardial ischemia)
Comparison
Abrupt occlusion of the left anterior descending… vs Baseline/control state
Design
Preclinical
Follow-up
22 minutes post-reperfusion
Authors
Discussion
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Implication
Phosphorus-31 NMR spectroscopy demonstrates that the intensity of ischemia correlates with reductions in regional contractile function and high-energy phosphates in a canine model.
Key Points
- To correlate rapid changes in regional myocardial blood flow and contractile function with high-energy phosphate metabolism during coronary occlusion and subsequent reperfusion.
- Subjected mongrel dogs to left anterior descending coronary artery occlusion for 17.1 ± 1.9 minutes followed by 22 minutes of reperfusion.
- Measured transmural myocardial blood flow using microspheres and regional contractile function with sonomicrometers.
- Quantified high-energy phosphate metabolites, including phosphocreatine and beta-ATP, via phosphorus-31 nuclear magnetic resonance spectroscopy at 4.4-minute sampling intervals.
- Transmural blood flow fell from 1.07 ± 0.25 to 0.25 ± 0.10 ml/(min · g), accompanied by holosystolic expansion as segmental systolic shortening decreased from 9.3 ± 3.7% to -6.3 ± 6.0%.
- Phosphocreatine dropped to 45.9 ± 17.0% of baseline by the end of occlusion, whereas beta-ATP reached its nadir (72.7 ± 13.3% of baseline) early after reperfusion.
- The phosphocreatine-to-inorganic phosphate ratio decreased from 3.34 ± 0.75 to 1.01 ± 0.61 during occlusion before recovering early in reperfusion, with ischemia intensity correlating significantly with contractile, metabolic, and pH declines.
Structured PICO
P
Population
Mongrel dogs subjected to abrupt occlusion of the left anterior descending coronary artery for ~17 minutes followed by reperfusion.
I
Intervention
Abrupt occlusion of the left anterior descending coronary artery for 17.1 +/- 1.9 minutes followed by complete release
C
Comparator
Baseline/control state
O
Outcome
Regional myocardial blood flow, function, and metabolism (high energy phosphate concentrations)surrogate
Phosphorus-31 NMR spectroscopy demonstrates that the intensity of ischemia correlates with reductions in regional contractile function and high-energy phosphates in a canine model.
Cite This Study
Guth et al. (1987) studied Regional myocardial ischemia and reperfusion. Coronary artery occlusion and reperfusion vs. Baseline/control was evaluated on Transmural blood flow, regional contractile function, and high energy phosphate concentrations. Coronary occlusion in dogs reduced transmural blood flow from 1.07 to 0.25 ml/(min X g) and segmental systolic shortening from 9.3% to -6.3%, correlating with reduced phosphocreatine levels.