Key result
Oxidative stress stalls yeast ribosomes at tryptophan codons, reducing translation elongation and inhibiting initiation.
Why the study?
Translational control is essential in response to stress, but translational programmes and the contribution of defence pathways launched upon environmental stresses required investigation.
Population
Wild type and defence pathway-inactivated fission yeast Schizosaccharomyces pombe cells
Comparison
Five environmental stresses vs each other or baseline in wild type vs defence pathway-inactivated cells
Design
Ribosome profiling preclinical study
Authors
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No immediate clinical implications from yeast model; leaves open conservation of ribosome stalling in mammalian oxidative stress responses.
The study reveals that oxidative stress specifically depletes charged tRNA-Tryptophan in fission yeast, causing ribosome stalling and queuing that contributes to translation initiation inhibition.
Rubio et al. (2020) studied Environmental stress in fission yeast. Environmental stresses vs. Unstressed cells / wild type vs defence pathway inactivated was evaluated on Translational programmes and ribosome stalling. Oxidative stress in fission yeast caused ribosome stalling on tryptophan codons and subsequent ribosome queuing, leading to reduced translation elongation and contributing to initiation inhibition.
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