What question did this study set out to answer?

To determine how rapeseed meal and its constituent compounds affect hepatic trimethylamine oxidase activity in laying hens susceptible to producing tainted eggs.

March 1, 1979Journal of the Science of Food and Agriculture

Effect of rapeseed meal on hepatic trimethylamine oxidase activity in the domestic fowl in relation to egg taint

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Authors

APA.W. PearsonUniversity of Edinburgh EBE.J. ButlerUniversity of London RCR. F. CurtisNorwich Research Park

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Implication

Controlled dietary trial reveals that rapeseed meal suppresses hepatic trimethylamine oxidase in susceptible hens, indicating dietary enzyme inhibition causes trimethylamine egg taint.

Key Points

To determine how rapeseed meal and its constituent compounds affect hepatic trimethylamine oxidase activity in laying hens susceptible to producing tainted eggs.
Shaver 585 hens classified as 'tainters' or 'non-tainters' were fed either a 10% Brassica napus rapeseed meal diet or an equivalent soya-bean and fish meal control diet for 16 weeks.
Hepatic microsomal trimethylamine (TMA) oxidase activity and apparent Michaelis constants were assayed ex vivo.
In vitro inhibition tests of hepatic TMA oxidase were conducted using sinapine, rapeseed acetone/methanol extracts, and cation exchange resin fractions.
Hepatic microsomal TMA oxidase activity was substantially lower and apparent Michaelis constants were elevated in hens fed rapeseed meal, with the lowest activity occurring in tainter hens.
Sinapine as well as acetone and methanol extracts from rapeseed meal strongly inhibited TMA oxidase in vitro, increasing apparent Michaelis constants roughly six-fold in both tainters and non-tainters.
Cation exchange separation of methanol extracts indicated the presence of an additional inhibitory compound in a low-sinapine fraction.

Cite This Study

Pearson et al. (1979) studied this question.

synapsesocial.com/papers/6a08f3a634cfc5f8bc5b823e https://doi.org/10.1002/jsfa.2740300314

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Implication

Controlled dietary trial reveals that rapeseed meal suppresses hepatic trimethylamine oxidase in susceptible hens, indicating dietary enzyme inhibition causes trimethylamine egg taint.

Effect of rapeseed meal on hepatic trimethylamine oxidase activity in the domestic fowl in relation to egg taint

Discussion

Member takes

Implication

Key Points

Cite This Study

Effect of rapeseed meal on hepatic trimethylamine oxidase activity in the domestic fowl in relation to egg taint

Discussion

Member takes

Implication

Key Points

Cite This Study

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