What question did this study set out to answer?

To review the implementation, applications, and technical limitations of atomic force microscopy-based single-cell force spectroscopy for quantifying cell adhesion.

May 20, 2008Journal of Cell Science

Single-cell force spectroscopy

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Authors

JHJonne HeleniusBoard of the Swiss Federal Institutes of Technology CHCarl‐Philipp HeisenbergInstitute of Science and Technology Austria HGHermann E. GaubMayo Clinic

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Implication

Review demonstrates atomic force microscopy for single-cell force spectroscopy under physiological conditions, highlighting molecular-level quantification of cell adhesion forces and kinetics.

Key Points

To review the implementation, applications, and technical limitations of atomic force microscopy-based single-cell force spectroscopy for quantifying cell adhesion.
Assessed atomic force microscopy (AFM) configurations adapted for single-cell force spectroscopy (SCFS).
Evaluated force actuation and measurement protocols applied to living cells under near-physiological conditions.
SCFS provides precise spatial and temporal control capable of measuring forces across whole cells down to individual adhesion-receptor-ligand interactions.
The technique resolves the mechanical forces, kinetics, and energetics governing cell-cell and cell-matrix adhesion under physiological conditions.

Cite This Study

Helenius et al. (2008) studied this question.

synapsesocial.com/papers/6a08f48f74a93f402dd38b59 https://doi.org/10.1242/jcs.030999

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Implication

Review demonstrates atomic force microscopy for single-cell force spectroscopy under physiological conditions, highlighting molecular-level quantification of cell adhesion forces and kinetics.

Single-cell force spectroscopy

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Implication

Key Points

Cite This Study

Single-cell force spectroscopy

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Member takes

Implication

Key Points

Cite This Study

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