Key result
Exposure of rat ventricular myocytes to PKA decreased Ca2+ sensitivity of isometric tension (pCa50 5.58 vs 5.74) but did not affect unloaded shortening velocity.
Population
Rat enzymatically isolated, tritonized ventricular myocytes
Comparison
Exposure to cAMP-dependent protein kinase in the… vs Before PKA treatment (baseline)
Design
Preclinical
Authors
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Does not support PKA-targeted therapies in HF; leaves open translation from rat myocytes to human myofilament function.
Absolute Event Rate: 5.58% vs 5.74%
In rat ventricular myocytes, PKA-induced phosphorylation of troponin I and C protein decreases Ca2+ sensitivity of isometric tension without affecting unloaded shortening velocity.
Hofmann et al. (1994) studied Rat cardiac myocytes. cAMP-dependent protein kinase (PKA) vs. Before PKA treatment was evaluated on Ca2+ sensitivity of isometric tension (pCa50). Exposure of rat ventricular myocytes to PKA decreased Ca2+ sensitivity of isometric tension (pCa50 5.58 vs 5.74) but did not affect unloaded shortening velocity.
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