What question did this study set out to answer?

Determine the primary N-glycan structures of major pollen and peanut allergens to evaluate how specific sugar residues influence recognition by IgE antibodies.

April 1, 2000Journal of Biological ChemistryOpen Access

β(1,2)-Xylose and α(1,3)-Fucose Residues Have a Strong Contribution in IgE Binding to Plant Glycoallergens

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Authors

RRRonald van ReeFlorida State University MCMarion Cabanes‐MacheteauPfizer (United States)JAJaap H. AkkerdaasAmsterdam University Medical Centers

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Implication

Biochemical analysis demonstrates distinct IgE binding to plant pollen and peanut glycoallergens, indicating that adjacent glycan branches sterically hinder xylose epitope recognition.

Key Points

Determine the primary N-glycan structures of major pollen and peanut allergens to evaluate how specific sugar residues influence recognition by IgE antibodies.
Determined the primary structures of N-glycans attached to pollen allergens (Lol p 11 and Ole e 1) and peanut allergen (Ara h 1).
Assessed IgE antibody binding via radioallergosorbent test (RAST) assays using protease digests of the three allergens alongside a panel of reference glycoproteins with characterized N-glycan profiles.
Both beta(1,2)-xylose and alpha(1,3)-fucose residues contribute significantly to IgE binding across plant glycoallergens.
Xylose-specific IgE antibodies that bound to Lol p 11 and bromelain failed to bind homologous xylose-containing glycans on horseradish peroxidase, phytohemagglutinin, Ole e 1, and Ara h 1.
Presence of an alpha(1,3)-mannose antenna on the core glycan sterically hinders IgE antibody access and binding to the beta(1,2)-xylose epitope.

Cite This Study

Ree et al. (2000) studied this question.

synapsesocial.com/papers/6a092e2efebbf018f816013f https://doi.org/10.1074/jbc.275.15.11451

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Implication

Biochemical analysis demonstrates distinct IgE binding to plant pollen and peanut glycoallergens, indicating that adjacent glycan branches sterically hinder xylose epitope recognition.

β(1,2)-Xylose and α(1,3)-Fucose Residues Have a Strong Contribution in IgE Binding to Plant Glycoallergens

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Key Points

Cite This Study

β(1,2)-Xylose and α(1,3)-Fucose Residues Have a Strong Contribution in IgE Binding to Plant Glycoallergens

Discussion

Member takes

Implication

Key Points

Cite This Study

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