Size-exclusion chromatography (SEC) of water-soluble polymers is a rapidly growing area, especially with the introduction of high-performance columns. The objective of this article is to review the state-of-the-art of aqueous SEC and to present a practical approach to methods development. Problems associated with aqueous SEC are reviewed in detail with emphasis on polyelectrolytes. A current listing of all commercially available packings for aqueous SEC is given along with brief descriptions of recently developed packings. Column calibration techniques for water-soluble polymers are briefly reviewed, including the use of a low-angle laser light-scattering detector. Finally, a selected bibliography of applications for SEC of water-soluble polymers is presented.
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Howard G. Barth (1980) studied this question.