Mitochondrial ROS scavenging with mitoTEMPO attenuated augmented RyR2-mediated sarcoplasmic reticulum calcium leak and proarrhythmic spontaneous calcium waves in ventricular myocytes.
Augmented RyR2 activity drives a proarrhythmic feedback cycle by modulating mitochondrial Ca2+ handling and promoting mitochondrial ROS emission, which further oxidizes RyR2.
Abstract Cardiac disease is associated with deleterious emission of mitochondrial reactive oxygen species (mito-ROS), as well as enhanced oxidation and activity of the sarcoplasmic reticulum (SR) Ca 2+ release channel, the ryanodine receptor (RyR2). The transfer of Ca 2+ from the SR via RyR2 to mitochondria is thought to play a key role in matching increased metabolic demand during stress. In this study, we investigated whether augmented RyR2 activity results in self-imposed exacerbation of SR Ca 2+ leak, via altered SR-mitochondrial Ca 2+ transfer and elevated mito-ROS emission. Fluorescent indicators and spatially restricted genetic ROS probes revealed that both pharmacologically and genetically enhanced RyR2 activity, in ventricular myocytes from rats and catecholaminergic polymorphic ventricular tachycardia (CPVT) mice, respectively, resulted in increased ROS emission under β-adrenergic stimulation. Expression of mitochondrial Ca 2+ probe mtRCamp1h revealed diminished net mitochondrial Ca 2+ with enhanced SR Ca 2+ leak, accompanied by depolarization of the mitochondrial matrix. While this may serve as a protective mechanism to prevent mitochondrial Ca 2+ overload, protection is not complete and enhanced mito-ROS emission resulted in oxidation of RyR2, further amplifying proarrhythmic SR Ca 2+ release. Importantly, the effects of augmented RyR2 activity could be attenuated by mitochondrial ROS scavenging, and experiments with dominant-negative paralogs of the mitochondrial Ca 2+ uniporter (MCU) supported the hypothesis that SR-mitochondria Ca 2+ transfer is essential for the increase in mito-ROS. We conclude that in a process whereby leak begets leak, augmented RyR2 activity modulates mitochondrial Ca 2+ handling, promoting mito-ROS emission and driving further channel activity in a proarrhythmic feedback cycle in the diseased heart.
Hamilton et al. (Fri,) conducted a other in Catecholaminergic polymorphic ventricular tachycardia (CPVT) (n=64). mitoTEMPO (mitochondrial ROS scavenging) vs. Control/Vehicle was evaluated on Sarcoplasmic reticulum Ca2+ leak and spontaneous Ca2+ wave latency. Mitochondrial ROS scavenging with mitoTEMPO attenuated augmented RyR2-mediated sarcoplasmic reticulum calcium leak and proarrhythmic spontaneous calcium waves in ventricular myocytes.