Measurement of the rate of protein synthesis and compartmentation of heart phenylalanine

Edward E. McKee; Joseph Y. Cheung; D. E. Rannels; Howard E. Morgan

doi:10.1016/s0021-9258(17)38107-3

February 1, 1978Journal of Biological ChemistryOpen Access

Measurement of the rate of protein synthesis and compartmentation of heart phenylalanine

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Key Points

Determine the accurate precursor pool for measuring myocardial protein synthesis rates and characterize the compartmentation of phenylalanine in the heart.
Perfused hearts with varying concentrations of [14C]phenylalanine (0.01 to 3.6 mM) and measured incorporation into myocardial protein.
Assayed specific radioactivity across extracellular, intracellular, and phenylalanyl-tRNA pools, with and without insulin addition.
Evaluated tracer equilibration kinetics and mathematically modeled amino acid compartmentation pathways.
At 0.01 mM perfusate phenylalanine, phenylalanyl-tRNA specific activity was 65% of extracellular and 155% of intracellular specific activity; using intracellular or extracellular pools alone distorted synthesis rate calculations.
At 0.4 mM perfusate phenylalanine, specific activities across extracellular, intracellular, and tRNA-bound pools equalized, yielding a protein synthesis rate of 106 nmol phenylalanine/g heart/h (matching the rate derived from phenylalanyl-tRNA at 0.01 mM).
Insulin doubled the protein synthesis rate (~100% increase) and accelerated isotopic equilibration without altering the relative specific activity relationships between precursor compartments.

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Implication

Ex vivo perfusion study uncovers tRNA aminoacylation from distinct precursor pools in heart tissue, indicating accurate synthesis rates require phenylalanyl-tRNA specific activity.

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Cite This Study

McKee et al. (1978) studied this question.

synapsesocial.com/papers/6a0933421d1abd907d16129d https://doi.org/10.1016/s0021-9258(17)38107-3

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Measurement of the rate of protein synthesis and compartmentation of heart phenylalanine

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Measurement of the rate of protein synthesis and compartmentation of heart phenylalanine

Key Points

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Implication

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