A Laboratory-Based Experimental Study on Ameliorative Effects of Vitamin D on Chronic Hepatitis Induced by Prolonged Use of Norethisterone in Female Rats

Objective: To assess the ameliorative effects of vitamin D on chronic hepatitis, which was induced by prolonged use of norethisterone in female rats.Study Design: A laboratory-based experimental animal study.Place and Duration of Study: The study was primarily conducted in the Department of Anatomy at the Army Medical College (AMC), Rawalpindi in association with the National Institute of Health (NIH) in Islamabad, Pakistan from August 2021 to November 2021.Methods: A total of thirty female Sprague-Dawley rats weighing between 250 and 300 grams were allocated into three groups randomly by the lottery method. There were ten animals in each group (N = 10 per group). Group A was a control group in which no intervention was carried out. For 8 weeks, Norethisterone at a dose of 4.55 mg/kg per day was administered orally via gavage to group B. Group C received the same dose of norethisterone for eight weeks, coupled with an intraperitoneal injection of vitamin D in a dose of 1000 IU/kg per day for five days a week. At the completion of the experiment, every animal was euthanized. H & E stainswere used to stain the liver specimens after they had been processed. All the specimens were evaluated histologically for focal, portal, and periportal hepatic inflammation.Results: Intergroup comparison produced the results. Significant focal, portal, and periportal hepatic inflammation was observed in experimental group B, with conclusions of 0.001, 0.001, and 0.033 (P-values). In experimental group C, there was significant improvement in hepatic inflammation, as indicated by P-values of 0.001, 0.001, and 0.029.Conclusion: Vitamin D has ameliorative effects on chronic hepatitis induced by prolonged use of norethisterone in female rats. How to cite this: Tabassum A, Kiani MRB, Zahid A, Khalid R, Bashir S, Iqbal S. A Laboratory-Based Experimental Study on AmeliorativeEffects of Vitamin D on Chronic Hepatitis Induced by Prolonged Use of Norethisterone in Female Rats. Life and Science. 2026; 7(2): 252-259. doi: http://doi.org/10.37185/LnS.1.1.789