Phenolic Hydroxyl Signal-Based Quantification of Polyphenolic Compounds by Quantitative ¹H NMR

April 5, 2026BUNSEKI KAGAKUOpen Access

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This research aims to utilize phenolic hydroxyl signals as reliable quantification markers for polyphenolic compounds in NMR analysis.
Investigated signal sharpening effects with metal salt (MgCl2) in DMSO-d6 solvent.
Analyzed isofloric acid, gallic acid, and rutin as models for phenolic compounds.
Measured and compared quantification values of Ar-OH signals with traditional C-H signals.
Significant signal sharpening for all Ar-OH signals observed with MgCl2 treatment.
Rutin showed stable observation of multiple Ar-OH and added OH signals from sugar chains.
Quantification values of Ar-OH closely matched C-H signal results, indicating high reproducibility.

Demonstrates improved quantification of polyphenolic compounds via phenolic hydroxyl signals, suggesting enhanced analytical accuracy.

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Kato et al. (2026) studied this question.

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