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Demonstrates controlled transcription and gene expression using orthogonal DNA timers, suggesting new strategies in synthetic biology.
) from 0.48 ± 0.02 h up to 8.4 ± 0.1 h. Using three different blocker-degrading enzymes (RNase H, uracil-DNA glycosylase (UDG), and formamidopyrimidine DNA glycosylase (Fpg)), we also demonstrate orthogonal temporal control of multiple transcription templates in a single solution. Finally, we show the programmed termination control and downstream regulation of Cas12a enzymatic collateral cleavage activity through such transcription timers. Together, these orthogonal DNA transcriptional timers establish a generalizable and straightforward framework for programming time-resolved transcription and gene expression in cell-free synthetic biology.
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Lerma-Escalera et al. (2026) studied this question.
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