What question did this study set out to answer?

Standardize an improved Swiss-rolling protocol to preserve the intact architecture of mouse intestinal and colonic tissues for downstream histological evaluation.

July 13, 2016Journal of Visualized ExperimentsOpen Access

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

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Authors

ABAgnieszka B. BialkowskaStony Brook University Hospital AGAmr M. GhalebCentre For Digestive Diseases MNMandayam O. NandanStony Brook University

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Implication

Methodological protocol demonstrates enhanced intestinal tissue preservation in mouse models, highlighting improved histological assessment of inflammation and tumorigenesis.

Key Points

Standardize an improved Swiss-rolling protocol to preserve the intact architecture of mouse intestinal and colonic tissues for downstream histological evaluation.
Optimized tissue harvesting, fixation, Swiss-rolling, and paraffin embedding reorientation procedures for mouse intestinal specimens.
Sectioned paraffin-embedded tissue rolls for comparative evaluation using nonfluorescent immunohistochemistry and multi-channel immunofluorescence.
The refined Swiss-rolling procedure preserved continuous epithelial morphology across the full length of the intestine, enabling comprehensive evaluation of inflammation and tumorigenesis.
Paraffin-embedded rolls supported clear cellular visualization through immunohistochemical staining and enabled multi-target intracellular co-localization using multi-channel immunofluorescence.

Cite This Study

Bialkowska et al. (2016) studied this question.

synapsesocial.com/papers/6a09b153a9b5885644345e59 https://doi.org/10.3791/54161

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Implication

Methodological protocol demonstrates enhanced intestinal tissue preservation in mouse models, highlighting improved histological assessment of inflammation and tumorigenesis.

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Discussion

Member takes

Implication

Key Points

Cite This Study

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Discussion

Member takes

Implication

Key Points

Cite This Study

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