Mitochondria as signaling organelles in the vascular endothelium

Vascular endothelial cells are highly glycolytic and consume relatively low amounts of oxygen (O 2 ) compared with other cells. We have confirmed that oxidative phosphorylation is not the main source of ATP generation in these cells. We also show that at a low O 2 concentration (<1%) endogenous NO plays a key role in preventing the accumulation of the α-subunit of hypoxia-inducible factor 1. At higher O 2 concentrations (1–3%) NO facilitates the production of mitochondrial reactive oxygen species. This production activates the AMP-activated protein kinase by a mechanism independent of nucleotide concentrations. Thus, the primary role of mitochondria in vascular endothelial cells may not be to generate ATP but, under the control of NO, to act as signaling organelles using either O 2 or O 2 -derived species as signaling molecules. Diversion of O 2 away from endothelial cell mitochondria by NO might also facilitate oxygenation of vascular smooth muscle cells.