What question did this study set out to answer?

To determine the metabolic requirements for extracellular asparagine uptake and endogenous asparagine synthetase (ASNS) expression during CD8+ T cell activation.

April 6, 2021JCI InsightOpen Access

Coordination of asparagine uptake and asparagine synthetase expression modulates CD8+ T cell activation

View Full Paper

Ask AI

Bookmark

To determine the metabolic requirements for extracellular asparagine uptake and endogenous asparagine synthetase (ASNS) expression during CD8+ T cell activation.
Stimulated CD8+ T cells in vitro via T cell receptor (TCR) engagement under conditions with or without extracellular asparagine.
Tracked cell viability, proliferation, metabolic reprogramming, and ASNS protein expression at early time points and beyond 24 hours.
Investigated the dependence of ASNS upregulation on TCR-induced mTOR signaling pathways.
Early during activation, CD8+ T cells lack significant ASNS expression, causing marked impairment of viability, growth, activation, and metabolic reprogramming when deprived of extracellular asparagine.
After 24 hours of stimulation, TCR-mediated mTOR signaling drives robust ASNS upregulation, enabling CD8+ T cells to survive and function independently of extracellular asparagine supplies.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

In vitro study demonstrates dynamic asparagine uptake and synthesis regulate CD8+ T cells, indicating coordinated metabolism drives optimal immune responses.

Cite This Study

Hope et al. (2021) studied this question.

synapsesocial.com/papers/6a09f8b300217ed3fb340ae5 https://doi.org/10.1172/jci.insight.137761

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates dynamic asparagine uptake and synthesis regulate CD8+ T cells, indicating coordinated metabolism drives optimal immune responses.

Coordination of asparagine uptake and asparagine synthetase expression modulates CD8+ T cell activation

Key Points

Discussion

Implication

Cite This Study

Coordination of asparagine uptake and asparagine synthetase expression modulates CD8+ T cell activation

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider