What is the clinical evidence from this study?

Study design: Cross-Sectional. Population: Bovine Enterovirus (n=103). Intervention: RT-PCR and sequencing. Primary outcome: Overall positivity for bovine enterovirus.

What does this research mean for the field?

Bovine enterovirus is present in 14.5% of tested cattle fecal samples in Brazil and phylogenetically clusters into the Enterovirus F clade. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

January 1, 2019Epidemiology and InfectionOpen Access

Detection and molecular characterisation of bovine Enterovirus in Brazil: four decades since the first report

Q: What are the key findings of this study?

Bovine enterovirus was detected in 14.5% of cattle fecal samples from Brazil, with phylogenetic analysis clustering the sequences into the Enterovirus F clade.

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Key result

Bovine enterovirus appears in ~15% of Brazilian cattle samples, clustering within the Enterovirus F clade.

n=103

Why the study?

Bovine enteroviruses are suggested to be involved in enteric infections, respiratory disease, reproductive disorders, and infertility, motivating updated detection and molecular characterisation in Brazil.

Population

103 faecal samples collected from the rectum of cattle in rural areas of the states of São Paulo, Minas…

Design

Preclinical

Authors

MCMarcelo CândidoUniversidade de São Paulo SASabrina Ribeiro de Almeida-QueirozUniversidade de São Paulo MBM.G. BuzinaroNúcleo de Pesquisas Aplicadas (Brazil)

Discussion

Member takes

Implication

Supports updated surveillance of bovine enteroviruses in Brazilian herds; leaves open their clinical impact and transmission dynamics.

Study Design

Type

Cross-Sectional (n=103)

Structured PICO

Population

103 faecal samples collected from the rectum of cattle (beef and dairy) in rural areas of the states of São Paulo, Minas Gerais, Goiás, Rio Grande do Sul, Paraná and Mato Grosso do Sul in Brazil. 36 (34.9%) had diarrhoea, 67 (65.0%) were aged <4 months, 71 (68.9%) were female. 74 (71.8%) feedlot, 29 (28.1%) free-range.

Intervention

RNA extraction, reverse transcription-polymerase chain reaction (RT-PCR) analysis, and partial sequencing of the 5'-terminal portion of bovine enterovirus (BEV)

Outcome

Detection rate and phylogenetic characterization of bovine enterovirus (BEV)

The study confirms the presence of bovine enterovirus in Brazilian cattle herds after four decades and characterizes them as belonging to the Enterovirus F clade.

Limitations

Cannot affirm that the viral strains detected trigger diarrhoea in infected animals or are prevalent in younger animals
Only four sequences showed quality for subsequent analyses
Cannot affirm that the viral strains detected trigger diarrhoea or are prevalent in younger animals
Future studies using specific primers for BEV-E or complete genome are required
Experimental infection of gnotobiotic cattle is necessary for a better understanding of severity

Cite This Study

Cândido et al. (2019) conducted a cross-sectional in Bovine Enterovirus (n=103). RT-PCR and sequencing was evaluated on Overall positivity for bovine enterovirus. Bovine enterovirus was detected in 14.5% of cattle fecal samples from Brazil, with phylogenetic analysis clustering the sequences into the Enterovirus F clade.

synapsesocial.com/papers/6a0a1de830285ee4a1342f12 https://doi.org/10.1017/s0950268818003394

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Detection and molecular characterisation of bovine Enterovirus in Brazil: four decades since the first report

Discussion

Member takes

Implication

Study Design

Structured PICO

Limitations

Cite This Study

Detection and molecular characterisation of bovine Enterovirus in Brazil: four decades since the first report

Discussion

Member takes

Implication

Study Design

Structured PICO

Limitations

Cite This Study

Also Consider

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