What question did this study set out to answer?

To clone the cDNA encoding human NMN adenylyltransferase and determine its chromosomal localization, tissue expression profile, and enzymatic properties.

January 1, 2001Journal of Biological ChemistryOpen Access

Molecular Cloning, Chromosomal Localization, Tissue mRNA Levels, Bacterial Expression, and Enzymatic Properties of Human NMN Adenylyltransferase

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Authors

MEMonica EmanuelliMarche Polytechnic University FCFrancesco CarnevaliMarche Polytechnic University FSFranca SaccucciMarche Polytechnic University

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Implication

Laboratory study reveals molecular characteristics and tissue distribution of human NMN adenylyltransferase, indicating its central metabolic role and therapeutic potential in cancer.

Key Points

To clone the cDNA encoding human NMN adenylyltransferase and determine its chromosomal localization, tissue expression profile, and enzymatic properties.
Isolated and sequenced full-length human NMN adenylyltransferase cDNA from a human placenta library using PCR primers based on tryptic peptide fragments.
Cloned the coding sequence into a pT7-7 vector to express recombinant enzyme in Escherichia coli, followed by chromatographic purification.
Evaluated mRNA expression profiles across diverse normal tissues and cancer cell lines using Northern blotting and mapped the gene locus.
Identified an 837-base pair open reading frame encoding a 31.9-kDa protein mapped to chromosome band 1p32–35.
Detected a major 3.1-kilobase and minor 4.1-kilobase transcript ubiquitously in human tissues, with markedly lower mRNA levels in cancer cell lines.
Achieved high-efficiency bacterial expression yielding 1.5 mg of functional recombinant enzyme per liter of culture medium.

Cite This Study

Emanuelli et al. (2001) studied this question.

synapsesocial.com/papers/6a0a4c9aac8a0d6c3ab4e72a https://doi.org/10.1074/jbc.m008700200

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Implication

Laboratory study reveals molecular characteristics and tissue distribution of human NMN adenylyltransferase, indicating its central metabolic role and therapeutic potential in cancer.

Molecular Cloning, Chromosomal Localization, Tissue mRNA Levels, Bacterial Expression, and Enzymatic Properties of Human NMN Adenylyltransferase

Discussion

Member takes

Implication

Key Points

Cite This Study

Molecular Cloning, Chromosomal Localization, Tissue mRNA Levels, Bacterial Expression, and Enzymatic Properties of Human NMN Adenylyltransferase

Discussion

Member takes

Implication

Key Points

Cite This Study

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