Key result
T2D upregulates coronary microvascular metabolic and adipogenic pathways in mouse spatial transcriptomic profiling.
Why the study?
To interrogate transcriptional differences potentially contributing to coronary microvascular disease in type 2 diabetes by profiling the coronary microcirculation and surrounding myocardium to identify new target pathways.
Identifies candidate pathways in diabetic CMD; hypothesis-generating and requires validation before any clinical relevance.
Coronary microvascular disease (CMD) is an early complication of type 2 diabetes (T2D) involving adverse endothelial and smooth muscle function, vascular remodeling, and alterations in mechanics. These culminate in impaired coronary blood flow. To interrogate transcriptional differences potentially contributing to CMD, we tested the hypothesis that comprehensive single-cell and spatial transcriptomic profiling of the coronary microcirculation and surrounding myocardium will identify new pathways to target in CMD. We utilized an innovative combination of single-cell RNA profiling and spatial transcriptomics to examine transcriptional differences and molecular signatures of CMD in T2D mice. Single-cell RNA profiling and spatial transcriptomics revealed an upregulation of genes linked to adipogenesis, fatty acid metabolism, and oxidative phosphorylation in T2D cell clusters and coronary microvascular-enriched regions. In ECs, VSMCs, cardiomyocyte clusters, fibroblasts, and macrophages, the upregulation of adipogenesis was directed by Angplt4 and Ephx2, whereas Hmgcs2 and Acot2 were the key players in the upregulation of fatty acid metabolism, and Pdk4 and Ech1 were the drivers of oxidative phosphorylation upregulation. These intriguing data support the well-documented concept that cardiac metabolic inflexibility in T2D heart failure-characterized by reduced mitochondrial function, increased reliance on fatty acid oxidation, and impaired glucose utilization-contributes to oxidative stress and lipotoxicity. Our data unveiled novel and unique gene expression signatures of coronary microvessels in the presence and absence of diabetes.
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McCallinhart et al. (2025) studied Type 2 diabetes with coronary microvascular disease. Type 2 diabetes vs. Absence of diabetes was evaluated on Transcriptional differences and molecular signatures of coronary microvascular disease. Single-cell and spatial transcriptomics in T2D mice revealed upregulation of genes linked to adipogenesis, fatty acid metabolism, and oxidative phosphorylation in coronary microvessels.
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