Key result
hiPSC-derived smooth muscle cell models advance disease modeling and therapeutic screening despite ongoing validation challenges.
Why the study?
Despite significant progress in using hiPSC-SMCs to model disease, challenges such as lack of specific validation markers and lack of methodological consensus remain.
This review summarizes the progress and ongoing challenges in using hiPSC-derived smooth muscle cells for disease modeling and therapeutic screening.
Answers are grounded in this paper and cite their sources.
Supports hiPSC-SMC models in vascular research; leaves open standardization before reliable therapeutic screening.
The implementation of human induced pluripotent stem cell (hiPSC) models has introduced an additional tool for identifying molecular mechanisms of disease that complement animal models. Patient-derived or CRISPR/Cas9-edited induced pluripotent stem cells differentiated into smooth muscle cells (SMCs) have been leveraged to discover novel mechanisms, screen potential therapeutic strategies, and model in vivo development. The field has evolved over almost 15 years of research using hiPSC-SMCs and has made significant strides toward overcoming initial challenges such as the lineage specificity of SMC phenotypes. However, challenges both specific (eg, the lack of specific markers to thoroughly validate hiPSC-SMCs) and general (eg, a lack of transparency and consensus around methodology in the field) remain. In this review, we highlight the recent successes and remaining challenges of the hiPSC-SMC model.
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Kwartler et al. (2024) conducted a review in Physiology and pathology modeling. Human induced pluripotent stem cell-derived smooth muscle cell (hiPSC-SMC) models was evaluated. Human induced pluripotent stem cell-derived smooth muscle cell models have advanced disease modeling and therapeutic screening, despite ongoing challenges in validation and methodology.
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