Transcriptomic Comparison of Human Peripartum and Dilated Cardiomyopathy Identifies Differences in Key Disease Pathways

Figures

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  Figure 1. Flow-diagram of differential gene expression profiles in PPCM and DCM heart failure.

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  Figure 2. Enriched pathways in PPCM (meta) and DCM. The top 20 pathways with FDR < 0.05 are sorted by fold enrichment. (A–D) shows enriched gene ontology biological processes (GO BP), and (E–H) shows enriched Reactome pathways.

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  Figure 3. Infiltrative immune cell populations in diseased and donor hearts derived from lymphoid lineages. Elevated natural killer (A) and memory CD4+ T cell (B) populations were identified in PPCM. In contrast, DCM had reduced levels of natural killer (A) and memory CD4+ T cell (B) populations. DCM patients also had elevated levels of CD8+ T Cells (C) and naïve b cells (D). Significance is denoted as * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data are presented as mean and standard error measurements.

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  Figure 4. Infiltrative immune cell populations in diseased and donor hearts derived from myeloid lineages. (A) M0 macrophage, (B) M1 macrophage, and (C) M2 macrophage populations differed in select disease states. (D) Both dendritic cells and (E) eosinophils both significantly differed in PPCM and DCM compared to donors. Significance is denoted as * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data are presented as mean and standard error measurements.

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  Figure 5. Heatmaps showing upregulated cytotoxic T cell genes in (A) PPCM and (B) DCM. DCM (purple), PPCM (green), and donor controls (orange).

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  Figure 6. Cell types in the LV myocardium of PPCM and DCM. (A) Cell-type composition deconvoluted from PPCM and DCM gene expression profiles using CibersortX. PPCM and DCM data are normalized to donor values. (B) Heatmap summarizing DEGs expressed in fibroblasts in PPCM (green), DCM (purple), and donors (orange). (C) Enrichment analysis summarizing the representative upregulated pathways and processes from the genes in (B). Significance is denoted as ** p < 0.01, *** p < 0.001, and **** p < 0.0001. Data presented as mean and standard error measurements.