What question did this study set out to answer?

To assess whether the cysteine-switch activation mechanism identified in human fibroblast collagenase serves as a universal regulatory principle for the entire matrix metalloproteinase family.

July 1, 1990Proceedings of the National Academy of SciencesOpen Access

The cysteine switch: a principle of regulation of metalloproteinase activity with potential applicability to the entire matrix metalloproteinase gene family.

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Authors

HWHarold E. Van WartImaTx (United States)HBHenning Birkedal‐HansenUniversity of Alabama at Birmingham

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Implication

Comparative review reveals a universal cysteine-zinc latency mechanism across matrix metalloproteinases, highlighting metabolic flexibility in physiological tissue remodeling.

Key Points

To assess whether the cysteine-switch activation mechanism identified in human fibroblast collagenase serves as a universal regulatory principle for the entire matrix metalloproteinase family.
Evaluated amino acid sequence homology across the propeptide and catalytic zinc-binding domains of all recognized matrix metalloproteinase family members.
Conducted a comparative literature synthesis of biochemical activation mechanisms observed across individual latent matrix metalloproteinases.
Identified strict sequence conservation flanking both the propeptide cysteine residue and catalytic histidine zinc-binding ligands across all matrix metalloproteinases.
Determined that dissociation of the intramolecular cysteine-zinc complex via multiple activation pathways uniformly relieves active-site inhibition across family members.

Cite This Study

Wart et al. (1990) studied this question.

synapsesocial.com/papers/6a0b2a4e4f5e7da68b2e2bb9 https://doi.org/10.1073/pnas.87.14.5578

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Implication

Comparative review reveals a universal cysteine-zinc latency mechanism across matrix metalloproteinases, highlighting metabolic flexibility in physiological tissue remodeling.

The cysteine switch: a principle of regulation of metalloproteinase activity with potential applicability to the entire matrix metalloproteinase gene family.

Discussion

Member takes

Implication

Key Points

Cite This Study

The cysteine switch: a principle of regulation of metalloproteinase activity with potential applicability to the entire matrix metalloproteinase gene family.

Discussion

Member takes

Implication

Key Points

Cite This Study

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