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Review emerging long-read sequencing technologies and computational assembly algorithms that enable the cost-effective generation of finished, gapless microbial genomes.

December 1, 2014Current Opinion in MicrobiologyOpen Access

One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly

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Review emerging long-read sequencing technologies and computational assembly algorithms that enable the cost-effective generation of finished, gapless microbial genomes.
Evaluated long-read sequencing platforms that generate individual reads spanning tens of kilobases in length.
Assessed computational assembly methods designed to resolve continuous, single-contig microbial chromosomes directly from long-read data.
Long-read sequencing technologies achieve automated microbial genome finishing at a cost below $1000 per genome.
Reads spanning tens of kilobases eliminate assembly fragmentation common to short-read methods, yielding gapless assemblies that improve reference databases and chromosomal structure studies.

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Implication

Methodological review demonstrates automated finishing of complete microbial genomes via long-read sequencing, highlighting improved reference databases and structural variation analysis.

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Koren et al. (2014) studied this question.

synapsesocial.com/papers/6a0b45344f5e7da68b2e32df https://doi.org/10.1016/j.mib.2014.11.014

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Implication

Methodological review demonstrates automated finishing of complete microbial genomes via long-read sequencing, highlighting improved reference databases and structural variation analysis.

One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly

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Implication

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One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly

Key Points

Discussion

Implication

Cite This Study

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