What question did this study set out to answer?

Review emerging long-read sequencing technologies and computational assembly algorithms that enable the cost-effective generation of finished, gapless microbial genomes.

December 1, 2014Current Opinion in MicrobiologyOpen Access

One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly

View Full Paper

Ask AI

Bookmark

Authors

SKSergey KorenNational Human Genome Research Institute APAdam M. PhillippyNational Human Genome Research Institute

Discussion

Member takes

Implication

Methodological review demonstrates automated finishing of complete microbial genomes via long-read sequencing, highlighting improved reference databases and structural variation analysis.

Key Points

Review emerging long-read sequencing technologies and computational assembly algorithms that enable the cost-effective generation of finished, gapless microbial genomes.
Evaluated long-read sequencing platforms that generate individual reads spanning tens of kilobases in length.
Assessed computational assembly methods designed to resolve continuous, single-contig microbial chromosomes directly from long-read data.
Long-read sequencing technologies achieve automated microbial genome finishing at a cost below $1000 per genome.
Reads spanning tens of kilobases eliminate assembly fragmentation common to short-read methods, yielding gapless assemblies that improve reference databases and chromosomal structure studies.

Cite This Study

Koren et al. (2014) studied this question.

synapsesocial.com/papers/6a0b45344f5e7da68b2e32df https://doi.org/10.1016/j.mib.2014.11.014

View Full Paper

Ask AI

Bookmark

Implication

Methodological review demonstrates automated finishing of complete microbial genomes via long-read sequencing, highlighting improved reference databases and structural variation analysis.

One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly

Discussion

Member takes

Implication

Key Points

Cite This Study

One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider