Human norovirus vaccination elicited antibodies, such as A1431, that exhibited broad blockade and neutralized the pandemic GII.P16-GII.4 Sydney strain by targeting conserved epitopes.
Does human norovirus vaccination elicit broad and pandemic strain neutralizing antibody responses?
Human norovirus vaccination elicits antibodies, such as A1431, capable of broad blockade and neutralization against pandemic strains through binding to conserved, partially exposed epitopes.
Rapidly evolving RNA viruses, such as the GII.4 strain of human norovirus (HuNoV), and their vaccines elicit complex serological responses associated with previous exposure. Specific correlates of protection, moreover, remain poorly understood. Here, we report the GII.4-serological antibody repertoire-pre- and post-vaccination-and select several antibody clonotypes for epitope and structural analysis. The humoral response was dominated by GII.4-specific antibodies that blocked ancestral strains or by antibodies that bound to divergent genotypes and did not block viral-entry-ligand interactions. However, one antibody, A1431, showed broad blockade toward tested GII.4 strains and neutralized the pandemic GII.P16-GII.4 Sydney strain. Structural mapping revealed conserved epitopes, which were occluded on the virion or partially exposed, allowing for broad blockade with neutralizing activity. Overall, our results provide high-resolution molecular information on humoral immune responses after HuNoV vaccination and demonstrate that infection-derived and vaccine-elicited antibodies can exhibit broad blockade and neutralization against this prevalent human pathogen.
Lindesmith et al. (Sat,) conducted a other in Human norovirus (HuNoV). Human norovirus (HuNoV) vaccination vs. Pre-vaccination was evaluated on Serological antibody repertoire and structural mapping of antibody clonotypes. Human norovirus vaccination elicited antibodies, such as A1431, that exhibited broad blockade and neutralized the pandemic GII.P16-GII.4 Sydney strain by targeting conserved epitopes.