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January 8, 2026Animal MicrobiomeOpen Access

Diet chemistry and cross-kingdom microbiota associate with black soldier fly larvae performance on regional side streams

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Authors

FGFriscasari F. GurusingaFraunhofer Institute for Molecular Biology and Applied EcologySHSabine HurkaLOEWE Centre for Translational Biodiversity GenomicsDKDaniel KreftFraunhofer Institute for Molecular Biology and Applied Ecology

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Overview

Feeding experiment reveals diet chemistry and gut microbiota modulate growth rates in black soldier fly larvae, highlighting agricultural side streams as viable substrates for insect farming.

Key Points

  • To determine how different regional agricultural side streams impact black soldier fly larval development, biomass yields, and gut microbial communities.
  • Reared black soldier fly larvae (Hermetia illucens) from hatching to pupation across five diets: control chicken feed, apple pomace, industrial-scale potato pulp, lab-scale potato pulp, and rapeseed cake.
  • Profiled larval gut microbial communities using 16S rRNA and ITS2 amplicon sequencing alongside functional microbial predictions and monitored L5-stage methane emissions.
  • Pupation time ranged from 25 days on rapeseed cake to 87 days on industrial potato pulp, with control chicken feed yielding a 7.6-fold higher biomass than apple pomace.
  • Larvae fed fiber-rich, nitrogen-poor diets showed an enrichment of predicted fiber-degrading and nitrogen-fixing gut bacteria, while no methane emissions were detected across any diet treatment.

Cite This Study

Gurusinga et al. (2026) studied this question.

synapsesocial.com/papers/6a0bb794aab637ffb5c201c1https://doi.org/10.1186/s42523-025-00509-6
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