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September 1, 1986The Journal of Experimental MedicineOpen Access

Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies.

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Authors

TMThomas R. MalekUniversity of MiamiGOG OrtegaBaylor College of MedicineCCChristina ChanChinese University of Hong Kong

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Implication

In vitro study reveals that crosslinked anti-Ly-6 antibodies trigger proliferation and interleukin-2 release in murine T cells, indicating Ly-6 functions in activation signaling.

Key Points

  • To determine whether cell-surface molecules encoded by the Ly-6 locus play a functional role in triggering or regulating murine T cell activation.
  • Treated murine T cell hybridomas and resting peripheral T cells with monoclonal antibody D7, a rat antibody recognizing a nonpolymorphic Ly-6 specificity.
  • Crosslinked the antibody using anti-immunoglobulin reagents or Fc receptor-bearing accessory cells in the presence or absence of phorbol myristate acetate, antigens, alloantigens, or mitogens.
  • Assayed interleukin-2 secretion and proliferative responses, comparing mAb D7 effects to other anti-Ly-6 monoclonal antibodies.
  • Crosslinked mAb D7 induced interleukin-2 secretion in T cell hybridomas and stimulated robust proliferation in resting peripheral T cells when co-stimulated with phorbol myristate acetate.
  • Addition of mAb D7 markedly augmented proliferative responses to antigen and alloantigen stimulation, whereas mitogen-driven proliferation remained unaffected.
  • Multiple additional monoclonal antibodies specific to Ly-6 locus products similarly provoked T cell proliferation following crosslinking in the presence of phorbol myristate acetate.

Cite This Study

Malek et al. (1986) studied this question.

synapsesocial.com/papers/6a0c43293b45b6e808886667https://doi.org/10.1084/jem.164.3.709
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