Detailed technical methods for the rapid laboratory production and validation of adeno-associated virus, retrovirus/lentivirus, and rabies virus constructs for neurobiology research are provided.
Provides detailed protocols for in-house production of viral vectors commonly used in neurobiology, offering a faster and cheaper alternative to commercial sources.
Advances in design and use of light-sensitive and light-emitting sensors have facilitated observation, measurement, and control of neuronal activities. Viruses are effective vectors for delivery of these valuable research tools to mammalian brains. Recombinant viruses are optimized to mediate regulatable, long-term, and cell-specific gene expression. Here, we describe production methods for three of the most commonly used types of recombinant viruses in neurobiology research: adeno-associated virus (AAV), retrovirus/lentivirus, and glycoprotein-deleted rabies virus. These viral constructs are frequently used for calcium imaging or to deliver neural tracers and optogenetic tools. Popular constructs are readily obtained commercially; however, customized virus production through commercial sources is time consuming and costly. This article aims to provide readers with detailed technical information for rapid production and validation of high-quality viral particles in a laboratory setting while highlighting advantages and limitations of each viral type. © 2019 by John Wiley & Sons, Inc.
Chen et al. (Mon,) reported a review. Viral constructs production was evaluated. Detailed technical methods for the rapid laboratory production and validation of adeno-associated virus, retrovirus/lentivirus, and rabies virus constructs for neurobiology research are provided.
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