The preparation of ⁵⁹ Fe-labelled transferrin for ferrokinetic studies

Serum labelled with 59 Fe for ferrokinetic studies should contain only [ 59 Fe] transferrin. [ 59 Fe] ferric citrate was incubated at 37°C for up to 24 hours with sera having an excess latent iron-binding capacity. The results showed that unbound [ 59 Fe] ferric citrate may be present for several hours. An anion exchange resin (IRA 400 Cl − ) column removed all the [ 59 Fe] ferric citrate and left only [ 59 Fe] transferrin in the eluate. It is suggested that all 59 Fe-labelled preparations for ferrokinetic studies should be passed through a resin column before injection.