Effect of Haematogenous oxidation therapy with Ultraviolet-C irridation in an alloxan-induced diabetes and a Poloxamer 407-induced Hyperlipidemia in rabbits

Background: Recently diabetes and hyperlipidemia (HL) considered a major source of mortality. Chemical treatments could minimize the symptoms but, still the disease exists. Previously Ultraviolet was usedfor treatment of ailments related to infection and metabolism. Methods:The studyevaluates the effects of Haematogenous Oxidation Therapy (HOT) on the blood when a low dose of Ultraviolet-C (UV-C) is directly irradiated to the blood in a diabetic rabbit model and to evaluate the effects of treatment on diabetic rabbit.Type 1 Diabetes and hyperlipidemia were induced by intravenous injection of alloxan monohydrate and subcutaneous injection of poloxamer 407,respectively. A 10 ml blood was collected from diabetic rabbits, blood was being perfused with oxygen for 10 seconds and UV-C was irradiated to the blood, UV-irradiated blood was transfused back to the original rabbits. The HOT treatment was performed a total of 10 times. It wasevaluated the effects of the HOT treatment on diabetes and HL through hematological and biochemical analyses before and after HOT treatment were performed. Results:Theresults indicated that the reduced body weight was increased and blood glucose levels were significantly reduced after the HOT treatment was performed when compared to those prior to the HOT treatment. In addition, CRE, BUN and UA levels indicating renal functions were significantly reduced when compared to those prior to the HOT treatment. When the HOT treatment was performed in a diabetic and HL rabbit model, our results indicate that blood glucose levels and lipids profile were improved. Conclusions:Biochemical and Hematological analyswas demonstrate that the HOT was effective to alleviate diabetes and HL.