What question did this study set out to answer?

To develop a rapid, streamlined microdevice method to isolate and detect circulating cancer-related exosomes directly from undiluted human plasma.

July 3, 2017ACS Nano

Rapid Isolation and Detection of Exosomes and Associated Biomarkers from Plasma

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To develop a rapid, streamlined microdevice method to isolate and detect circulating cancer-related exosomes directly from undiluted human plasma.
Applied an alternating current electrokinetic (ACE) microarray chip device to 30–50 μL of undiluted human plasma containing glioblastoma exosomes.
Concentrated nanoscale exosomes into high-field regions around microelectrodes within 15 minutes followed by a buffer wash to remove bulk plasma components.
Performed downstream on-chip immunofluorescence analysis and extracted mRNA for reverse transcription polymerase chain reaction (RT-PCR).
The ACE microchip concentrated exosomes into electrode regions within 15 minutes, completing the full isolation and on-chip fluorescence detection protocol in under 30 minutes.
Isolated glioblastoma exosomes yielded viable mRNA suitable for subsequent RT-PCR verification alongside successful on-chip protein biomarker detection.

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Implication

Microfluidic assay demonstrates rapid isolation of glioblastoma exosomes from undiluted human plasma, suggesting potential for point-of-care liquid biopsy.

Cite This Study

Ibsen et al. (2017) studied this question.

synapsesocial.com/papers/6a0cbc37fa740485438414ca https://doi.org/10.1021/acsnano.7b00549

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Implication

Microfluidic assay demonstrates rapid isolation of glioblastoma exosomes from undiluted human plasma, suggesting potential for point-of-care liquid biopsy.

Rapid Isolation and Detection of Exosomes and Associated Biomarkers from Plasma

Key Points

Discussion

Implication

Cite This Study

Rapid Isolation and Detection of Exosomes and Associated Biomarkers from Plasma

Key Points

Discussion

Implication

Cite This Study

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