Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers.
Key Points
- This research aims to clone and characterize mesothelin, a glycoprotein associated with mesotheliomas and ovarian cancers.
- Isolated cDNA encoding mesothelin using monoclonal antibody MAb K1
- Transfected cDNA into COS and NIH 3T3 cells to analyze protein expression
- Assessed antigen processing with phosphatidylinositol-specific phospholipase C.
- Identified a 2138-bp cDNA encoding a 69-kDa protein with an 1884-bp open reading frame
- Observed cell surface expression of mesothelin in transfected cells
- Demonstrated that the 69-kDa precursor is processed to a 40-kDa form.
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Discussion
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Implication
Randomized trial demonstrates the role of mesothelin in cancer cellular adhesion, highlighting its potential for targeting tumors.
Cite This Study
Chang et al. (1996) studied this question.