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This research aims to purify and characterize a colony-stimulating factor from mouse lung-conditioned medium.

Purification and properties of colony-stimulating factor from mouse lung-conditioned medium.

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This research aims to purify and characterize a colony-stimulating factor from mouse lung-conditioned medium.
Purification of colony-stimulating factor from lung-conditioned medium by a 3500-fold process.
Analysis through polyacrylamide gel electrophoresis to confirm molecular weight and protein characteristics.
Investigation of factors affecting the stability and activity of colony-stimulating factor during purification.
A single protein component with a molecular weight around 29,000 as per gel filtration.
High concentrations (greater than 20 mug/ml) stimulated mixed colonies, while lower concentrations (less than 70 pg/ml) only stimulated macrophage colonies.
Colony-stimulating factor exhibited glycoprotein characteristics by binding to concanavalin A-Sepharose.

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Purified colony-stimulating factor boosts bone marrow cell growth, indicating therapeutic potential in immune responses.

Burgess et al. (1977) studied this question.

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