What question did this study set out to answer?

Characterize the cellular specificity and diagnostic utility of the novel monoclonal antibody Y1/82A across human tissues and neoplastic conditions.

July 1, 1988Journal of Clinical PathologyOpen Access

Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages.

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Authors

FDFrederick R. DaveyNHS Fife JCJ L CordellJohn Radcliffe Hospital WEWendy N. ErberThe University of Western Australia

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Implication

Laboratory study demonstrates selective binding of monoclonal antibody Y1/82A to monocytes and tissue macrophages, highlighting its diagnostic potential in monocytic leukemias.

Key Points

Characterize the cellular specificity and diagnostic utility of the novel monoclonal antibody Y1/82A across human tissues and neoplastic conditions.
Raised monoclonal antibody Y1/82A against phytohaemagglutinin-activated human peripheral blood mononuclear cells.
Assessed cellular reactivity and antigen localization using immunohistochemical staining and Western blot analysis across normal tissues and various malignant neoplasms.
Y1/82A reacted specifically with peripheral blood monocytes, bone marrow monocytes, resident tissue macrophages, osteoclasts, and megakaryocytes, while remaining unreactive with other normal cell types.
Positive binding occurred in malignant cells from acute myelomonocytic and acute monocytic leukemias, with no reactivity observed in malignant lymphoproliferative disorders or epithelial tumors.
Western blot analysis localized the target antigen to intracellular granules within macrophages.

Cite This Study

Davey et al. (1988) studied this question.

synapsesocial.com/papers/6a0ccbbfa4d785ea816259c0 https://doi.org/10.1136/jcp.41.7.753

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Implication

Laboratory study demonstrates selective binding of monoclonal antibody Y1/82A to monocytes and tissue macrophages, highlighting its diagnostic potential in monocytic leukemias.

Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages.

Discussion

Member takes

Implication

Key Points

Cite This Study

Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages.

Discussion

Member takes

Implication

Key Points

Cite This Study

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