The aim is to develop a straightforward method for preparing high specific activity 32P-labelled ATP.
Introduced a simplified procedure for the preparation of 32P-labelled ATP.
Compared new method to existing chemical methods, which are noted to be tedious.
New method produces 32P-labelled ATP of comparable quality to existing methods.
High specific activity achieved, enhancing utility in biochemical applications.
Abstract
32P-labelled ATP of high specific activity is not available commercially.Chemical methods for preparing it (see, for example, Lowenstein & Metzenberg, 1960) are tedious and yield a product