Effects of hematopoietic growth factors on purified bone marrow progenitor cells

We have used highly enriched hematopoietic progenitor cells and in-vitro culture to examine the questions:1. The effects of recombinant lL-3 and GM-CSF on proliferation and differentiation of enriched progenitor cells have not been clearly defined:- how do IL~3 and GM~CSF compare with respect to number and types of colonies induced?-to what extent do accessory cells influence colony formation induced by IL-3 and GM~CSF?{chapters 2 and 3)2. The effects of recombinant G-CSF, M~CSF and IL-6 on enriched hematopoietic progenitor in connection with IL-3 and GM~CSF have not been fully elucidated:~ what is the role of synergistic effects between G~CSF, M-CSF and IL~6 on the one hand IL~3 and GM~CSF on the other hand, on the proliferation and differentiation of colony cells?(chapters 4, 5 and 6)3. Consistent results on the effects of ll-1 on proliferation of immature bone marrow cells are:~does ll-1 directly induce proliferation?-what is the role of GM~CSF and TNF as mediators of the IL-1 effect?(chapter 7)22