DNA-Dependent DNA Polymerases I and II from Normal Human-Blood Lymphocytes
Key result
DNA polymerases I and II purified from normal human lymphocytes do not transcribe heteropolymeric regions of tumor-virus RNA into DNA, unlike DNA polymerase from leukemic lymphoblasts.
Population
Normal human-blood lymphocytes and lymphoblasts from individuals with leukemia
Comparison
Purification and characterization of… vs DNA polymerase isolated from lymphoblasts from…
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Suggests leukemia-specific polymerase activity; leaves open diagnostic or therapeutic targeting in leukemia.
Structured PICO
P
Population
Normal human-blood lymphocytes and lymphoblasts from individuals with leukemia
I
Intervention
Purification and characterization of DNA-dependent DNA polymerases I and II
C
Comparator
DNA polymerase isolated from lymphoblasts from individuals with leukemia
O
Outcome
Transcription of heteropolymeric regions of tumor-virus RNA into DNA
DNA polymerases from normal human lymphocytes do not transcribe tumor-virus RNA into DNA, unlike those from leukemia lymphoblasts.
Cite This Study
Smith et al. (1972) studied this question. DNA polymerases I and II purified from normal human lymphocytes do not transcribe heteropolymeric regions of tumor-virus RNA into DNA, unlike DNA polymerase from leukemic lymphoblasts.