Key result
Working at -20°C in 40% ethylene glycol resulted in a 1000-fold decrease in actomyosin ATPase activity and an increase in activation energy.
Subzero temperatures with ethylene glycol can stabilize reaction intermediates on the actomyosin pathway by drastically reducing ATPase activity.
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May enable trapping of actomyosin intermediates in vitro; leaves open relevance to cardiac muscle kinetics.
Travers et al. (1979) studied Myosin subfragment 1 ATPase activity. Subzero conditions with ethylene glycol vs. 20°C was evaluated on ATPase activities. Working at -20°C in 40% ethylene glycol resulted in a 1000-fold decrease in actomyosin ATPase activity and an increase in activation energy.
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