What question did this study set out to answer?

To characterize the spontaneous reversibility of noradrenergic and mesenchymal cellular identities and evaluate how the microenvironment influences tumor cell plasticity in neuroblastoma.

May 4, 2023Nature CommunicationsOpen Access

Reversible transitions between noradrenergic and mesenchymal tumor identities define cell plasticity in neuroblastoma

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Authors

CTCécile ThirantInserm APAgathe PeltierInstitut Gustave Roussy SDSimon DurandUniversité Claude Bernard Lyon 1

Discussion

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Implication

Preclinical study uncovers reversible transitions between noradrenergic and mesenchymal states in neuroblastoma, indicating that microenvironmental cues govern tumor cell plasticity.

Key Points

To characterize the spontaneous reversibility of noradrenergic and mesenchymal cellular identities and evaluate how the microenvironment influences tumor cell plasticity in neuroblastoma.
Assessed spontaneous phenotype switching and epigenetic reprogramming across multiple in vitro neuroblastoma cell line models and in vivo xenografts.
Performed single-cell RNA-sequencing on 18 human neuroblastoma tumor biopsies and 15 patient-derived xenograft (PDX) models to evaluate identity profiles in vivo.
Neuroblastoma cells displayed spontaneous and reversible transitions between noradrenergic and mesenchymal identities coupled with epigenetic reprogramming.
In vivo xenografts derived from either cell identity converged toward a noradrenergic phenotype, demonstrating strong microenvironmental selection pressure.
Single-cell RNA-seq confirmed predominant noradrenergic profiles across all 18 patient biopsies and 15 PDX models, while revealing a distinct noradrenergic subpopulation with mesenchymal features.

Cite This Study

Thirant et al. (2023) studied this question.

synapsesocial.com/papers/6a0cd46fa4d785ea816261e3 https://doi.org/10.1038/s41467-023-38239-5

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May 4, 2023Nature CommunicationsOpen Access

Reversible transitions between noradrenergic and mesenchymal tumor identities define cell plasticity in neuroblastoma

View Full Paper

Ask AI

Bookmark

Authors

CTCécile ThirantInserm APAgathe PeltierInstitut Gustave Roussy SDSimon DurandUniversité Claude Bernard Lyon 1

Discussion

Member takes

Implication

Preclinical study uncovers reversible transitions between noradrenergic and mesenchymal states in neuroblastoma, indicating that microenvironmental cues govern tumor cell plasticity.

Key Points

To characterize the spontaneous reversibility of noradrenergic and mesenchymal cellular identities and evaluate how the microenvironment influences tumor cell plasticity in neuroblastoma.
Assessed spontaneous phenotype switching and epigenetic reprogramming across multiple in vitro neuroblastoma cell line models and in vivo xenografts.
Performed single-cell RNA-sequencing on 18 human neuroblastoma tumor biopsies and 15 patient-derived xenograft (PDX) models to evaluate identity profiles in vivo.
Neuroblastoma cells displayed spontaneous and reversible transitions between noradrenergic and mesenchymal identities coupled with epigenetic reprogramming.
In vivo xenografts derived from either cell identity converged toward a noradrenergic phenotype, demonstrating strong microenvironmental selection pressure.
Single-cell RNA-seq confirmed predominant noradrenergic profiles across all 18 patient biopsies and 15 PDX models, while revealing a distinct noradrenergic subpopulation with mesenchymal features.

Cite This Study

Thirant et al. (2023) studied this question.

synapsesocial.com/papers/6a0cd46fa4d785ea816261e3 https://doi.org/10.1038/s41467-023-38239-5

Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

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