What question did this study set out to answer?

To determine whether the primary microRNA transcript BIC and its mature derivative miR-155 are expressed and processed across Hodgkin lymphoma and specific non-Hodgkin B-cell lymphoma subtypes.

July 22, 2005The Journal of PathologyOpen Access

BIC and miR‐155 are highly expressed in Hodgkin, primary mediastinal and diffuse large B cell lymphomas

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Authors

JKJoost KluiverUniversity Medical Center Groningen SPSibrand PoppemaUniversity Medical Center Groningen DJDebora de JongUniversity Medical Center Groningen

Discussion

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Implication

Laboratory study reveals elevated BIC and miR-155 expression in aggressive B-cell lymphomas, indicating active microRNA processing in distinct tumor subtypes.

Key Points

To determine whether the primary microRNA transcript BIC and its mature derivative miR-155 are expressed and processed across Hodgkin lymphoma and specific non-Hodgkin B-cell lymphoma subtypes.
Assessed BIC and mature miR-155 expression in Hodgkin lymphoma cell lines and tissue specimens.
Screened non-Hodgkin subtypes, including primary mediastinal B-cell lymphoma (N=8) and diffuse large B-cell lymphoma (N=18), using RNA in situ hybridization, qRT-PCR, and Northern blot analysis.
High levels of mature miR-155 were confirmed in Hodgkin lymphoma cell lines and tissues, demonstrating that the primary BIC transcript is actively processed into microRNA.
Significant BIC expression was detected in 12 of 18 diffuse large B-cell lymphoma cases and 8 of 8 primary mediastinal B-cell lymphoma cases, while remaining absent in most other non-Hodgkin subtypes.
Tumor cells with an activated B-cell-like phenotype showed higher proportions of BIC positivity than those with a germinal centre B-cell-like phenotype, with mature miR-155 detected across all tested cell lines and tissue samples.

Cite This Study

Kluiver et al. (2005) studied this question.

synapsesocial.com/papers/6a0cd541c6fb28010734cc71 https://doi.org/10.1002/path.1825

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Implication

Laboratory study reveals elevated BIC and miR-155 expression in aggressive B-cell lymphomas, indicating active microRNA processing in distinct tumor subtypes.

BIC and miR‐155 are highly expressed in Hodgkin, primary mediastinal and diffuse large B cell lymphomas

Discussion

Member takes

Implication

Key Points

Cite This Study

BIC and miR‐155 are highly expressed in Hodgkin, primary mediastinal and diffuse large B cell lymphomas

Discussion

Member takes

Implication

Key Points

Cite This Study

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