T-type Ca2+ channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast

Figures

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  Figure 1. Classification of histological types. Mammary glands, stained with hematoxylin and eosin (400× magnification, scale bar, 50 µm). A, Breast without alteration; (B) ductal hyperplasia; (C) ductal carcinoma in situ; (D) invasive ductal carcinoma.

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  Figure 2. Human mammary glands with immunohistochemical staining for CaV3.1 identified by DAB chromogen staining and hematoxylin counterstaining (main image 400× magnification, insert 600× magnification; scale bar, 50 µm). A, Breast without alteration showing nuclear expression (arrow) and absence of cytoplasmic immunostaining. B, Ductal hyperplasia with nuclear immunostaining (arrow). C, Ductal carcinoma in situ showing decreased nuclear expression and presence of cytoplasmic expression (arrow). D, Invasive ductal carcinoma with few nuclear immunostainings and cytoplasmic expression (arrow). Statistical test: Pearson’s correlation coefficient.

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  Figure 3. Human mammary glands with immunohistochemical staining for CaV3.2 identified by DAB chromogen staining and hematoxylin counterstaining (main image 400× magnification and insert 600× magnification; scale bar, 50 µm). A, Breast without alteration showing nuclear expression (arrow) and no cytoplasmic immunostaining. B, Ductal hyperplasia with nuclear expression (arrow). C, Ductal carcinoma in situ showing decreased nuclear expression and cytoplasmic immunostaining (arrow). D, Invasive ductal carcinoma with few nuclear immunostainings and cytoplasmic expression (arrow). Statistical test: Pearson’s correlation coefficient.

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  Figure 4. Human mammary glands with immunohistochemical staining for CaV3.3 identified by DAB chromogen staining and hematoxylin counterstaining (main image 400× and insert 600× magnification; scale bar, 50 µm). A, Breast without alteration showing nuclear expression (arrow) and no cytoplasmic immunostaining. B, Ductal hyperplasia with nuclear expression (arrow). C, Ductal carcinoma in situ showing decreased nuclear expression and cytoplasmic expression (arrow). D, Invasive ductal carcinoma with few nuclear immunostainings and cytoplasmic expression (arrow). Statistical test: Pearson’s correlation coefficient.