Evidence for biased bidirectional polymerization of actin filaments using heavy meromyosin prepared by an improved method.
Key result
Actin filaments decorated with heavy meromyosin grow by adding monomers to either end, with a bias toward the end normally attached to the Z line in striated muscle.
Population
Isolated actin filaments decorated with heavy meromyosin (HMM)
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May inform sarcomere models in cardiac muscle; leaves open translation to human disease or therapy.
Structured PICO
P
Population
Isolated actin filaments decorated with heavy meromyosin (HMM)
I
Intervention
Addition of actin monomers
O
Outcome
Directional bias of actin filament polymerization/growth
Demonstrates biased bidirectional polymerization of actin filaments, favoring the Z-line attached end.
Cite This Study
Woodrum et al. (1975) studied this question. Actin filaments decorated with heavy meromyosin grow by adding monomers to either end, with a bias toward the end normally attached to the Z line in striated muscle.