Key result
The presence of K+ and/or Mg2+ ions converted G-actin into an activated form with a life-time of about 8 min or less, detected by increased accessibility of thiol groups of Cys-217 and Cys-374.
Population
Globular actin (G-actin) molecules in vitro
Comparison
Addition of K+ and/or Mg2+ ions vs Unactivated G-actin
Design
Preclinical
Authors
Loading...
No immediate clinical implications; leaves open physiological relevance of short-lived activated G-actin.
The study provides direct chemical evidence for a short-lived, activated form of G-actin during polymerization, characterized by increased accessibility of specific thiol groups.
Shu et al. (1992) studied Actin polymerization. K+ and/or Mg2+ ions was evaluated on Accessibility of free thiol groups on G-actin. The presence of K+ and/or Mg2+ ions converted G-actin into an activated form with a life-time of about 8 min or less, detected by increased accessibility of thiol groups of Cys-217 and Cys-374.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: