What question did this study set out to answer?

To characterize conserved amino acid sequence elements and spacing constraints within the GTP-binding domain across diverse protein families.

April 1, 1987Proceedings of the National Academy of SciencesOpen Access

GTP-binding domain: three consensus sequence elements with distinct spacing.

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Population

Protein sequence data base of the Protein Identification Resource and known GTP-binding proteins

Design

Preclinical

Authors

TDThomas DeverNational Institutes of Health MGManuel J. GlyniasRoswell Park Comprehensive Cancer Center WMWilliam C. MerrickEngelhardt Institute of Molecular Biology

Discussion

Member takes

Implication

The GTP-binding consensus sequence may enable functional annotation of novel proteins; leaves open experimental validation in disease models.

Key Points

To characterize conserved amino acid sequence elements and spacing constraints within the GTP-binding domain across diverse protein families.
Analyzed and compared amino acid sequences across nine functionally distinct families of GTP-binding proteins.
Screened the Protein Identification Resource (PIR) database using the defined tripartite consensus sequence and spacing rules.
Established a consensus motif of three elements (GXXXXGK, DXXG, and NKXD) with conserved spacings of 40–80 or 130–170 residues between elements 1 and 2, and 40–80 residues between elements 2 and 3.
Identified that replacing NKXD with NKXW confers the ability to utilize ITP in addition to GTP, while dGTP functions with efficiency comparable to GTP.
Database screening matched known GTP-binding proteins alongside protein 2C of foot-and-mouth disease virus, predicting nucleotide binding in 2C proteins of related picornaviruses.

Structured PICO

Population

Protein sequence data base of the Protein Identification Resource (PIR, release 10.0, ~3800 protein sequences) and known GTP-binding proteins

Intervention

Sequence comparison and database screening for three consensus elements (GXXXXGK, DXXG, and NKXD) with specific spacing

Outcome

Identification of proteins matching the GTP-binding domain consensus sequencesurrogate

The identification of a specific consensus sequence for GTP-binding domains allows for the prediction of GTP-binding function in newly sequenced proteins, such as viral protein 2C.

Cite This Study

Dever et al. (1987) studied this question.

synapsesocial.com/papers/6a0ce1f3df69d124d80266ba https://doi.org/10.1073/pnas.84.7.1814

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Implication

The GTP-binding consensus sequence may enable functional annotation of novel proteins; leaves open experimental validation in disease models.

GTP-binding domain: three consensus sequence elements with distinct spacing.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

GTP-binding domain: three consensus sequence elements with distinct spacing.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider