What question did this study set out to answer?

To characterize the morphological features and functional electrolyte transport properties of the human colonic carcinoma cell line T84.

February 1, 1984AJP Gastrointestinal and Liver Physiology

A human colonic tumor cell line that maintains vectorial electrolyte transport

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Authors

KDK. DharmsathaphornUniversity of San Diego JMJames A. McRobertsUniversity of California, Los Angeles KMKenneth G. MandelSouthern Illinois University Carbondale

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Implication

In vitro study reveals polarized structure and regulated vectorial electrolyte transport in T84 colonic carcinoma cells, indicating their utility as an intestinal epithelial model.

Key Points

To characterize the morphological features and functional electrolyte transport properties of the human colonic carcinoma cell line T84.
Cultured T84 human colonic carcinoma cells to confluence on culture plates.
Evaluated monolayer morphology, membrane polarity, and intercellular junctions.
Measured vectorial electrolyte transport responses following exposure to secretagogues and antisecretagogues.
Cells formed polarized monolayers displaying an attached basolateral membrane, an apical membrane with microvilli, desmosomes, and tight junctions.
Confluent monolayers demonstrated directional electrolyte transport that was modulated by secretagogues and antisecretagogues similarly to intact intestinal tissue.

Cite This Study

Dharmsathaphorn et al. (1984) studied this question.

synapsesocial.com/papers/6a0ce24b004017e1a0fc0ca0 https://doi.org/10.1152/ajpgi.1984.246.2.g204

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Implication

In vitro study reveals polarized structure and regulated vectorial electrolyte transport in T84 colonic carcinoma cells, indicating their utility as an intestinal epithelial model.

A human colonic tumor cell line that maintains vectorial electrolyte transport

Discussion

Member takes

Implication

Key Points

Cite This Study

A human colonic tumor cell line that maintains vectorial electrolyte transport

Discussion

Member takes

Implication

Key Points

Cite This Study

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