Microscopy study reveals dipicolinic acid localization within dormant Bacillus subtilis spore cores, highlighting molecular mechanisms underlying bacterial dormancy and heat resistance.
The localization of dipicolinic acid in dormant spores of Bacillus subtilis was examined by an immunoelectron microscopy method with colloidal gold-immunoglobulin G complex. The colloidal gold particles were distributed mainly in the core regions of dormant spores and were not observed in those of germinated or autoclaved spores. This result clearly demonstrates that dipicolinic acid is localized in the cores of dormant spores.
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Kozuka et al. (1985) studied this question.
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