FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA
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Key result
Recombinant plasmid pFBJ-2 containing the entire FBJ-MSV proviral DNA induced morphological transformation of rat fibroblasts in tissue culture.
Population
Preclinical models including FBJ-MSV-transformed nonproducer rat cells, uninfected cells, and DNA from…
Comparison
Molecular cloning of FBJ-MSV proviral DNA and… vs Uninfected cells or carrier DNA transfection.
Design
Preclinical
Authors
Discussion
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Implication
No immediate clinical implications; leaves open c-fos functional roles in vertebrate oncogenesis.
Structured PICO
P
Population
Preclinical models including FBJ-MSV-transformed nonproducer rat cells (208F, NRK), uninfected cells, and DNA from various mammalian species (mouse, human, mink, chicken).
I
Intervention
Molecular cloning of FBJ-MSV proviral DNA (pFBJ-2) and transfection into rat fibroblasts.
C
Comparator
Uninfected cells or carrier DNA transfection.
O
Outcome
Identification, molecular cloning, and biological activity (morphological transformation) of FBJ-MSV proviral DNA, and identification of the v-fos gene and its cellular homolog (c-fos).
The study successfully cloned the biologically active FBJ-MSV provirus and identified the v-fos oncogene along with its conserved cellular homolog (c-fos) in normal vertebrate genomes.
Cite This Study
Curran et al. (1982) studied FBJ murine osteosarcoma virus. Molecular cloning of FBJ-MSV proviral DNA was evaluated on Identification and cloning of FBJ-MSV proviral DNA and transformation of rat fibroblasts. Recombinant plasmid pFBJ-2 containing the entire FBJ-MSV proviral DNA induced morphological transformation of rat fibroblasts in tissue culture.
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