What question did this study set out to answer?

To identify the specific signaling protein binding sites necessary for the endocytic internalization and intracellular trafficking of activated platelet-derived growth factor receptors.

February 4, 1994Science

Disruption of PDGF Receptor Trafficking by Mutation of Its PI-3 Linase Binding Sites

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Authors

MJMarguerite JolySanofi (France)AKAndrius KazlauskasUniversity of Illinois Urbana-Champaign FFFredric S. FayAlbert Einstein College of Medicine

Discussion

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Implication

In vitro experiment reveals that PI-3 kinase binding sites drive internal PDGF receptor trafficking in human cells, indicating their essential role in receptor endocytosis.

Key Points

To identify the specific signaling protein binding sites necessary for the endocytic internalization and intracellular trafficking of activated platelet-derived growth factor receptors.
Expressed wild-type and mutant human PDGF receptors in human Hep G2 cells and stimulated them with PDGF.
Assessed receptor localization using an F5 mutant lacking binding sites for multiple SH2 domain-containing proteins, an F5 mutant with restored PI-3 kinase sites, and a mutant selectively lacking only PI-3 kinase binding sites.
Wild-type PDGF receptors internalized and concentrated juxtanuclearly near the Golgi network within 10 minutes of PDGF treatment, whereas the F5 mutant remained localized at the cell periphery.
Restoration of PI-3 kinase binding sites alone on the F5 mutant fully rescued intracellular concentration, while selective deletion of only PI-3 kinase binding sites prevented concentration, showing these sites are necessary and sufficient for trafficking.

Cite This Study

Joly et al. (1994) studied this question.

synapsesocial.com/papers/6a0d32b136162e2f4b09a78a https://doi.org/10.1126/science.8303278

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Implication

In vitro experiment reveals that PI-3 kinase binding sites drive internal PDGF receptor trafficking in human cells, indicating their essential role in receptor endocytosis.

Disruption of PDGF Receptor Trafficking by Mutation of Its PI-3 Linase Binding Sites

Discussion

Member takes

Implication

Key Points

Cite This Study

Disruption of PDGF Receptor Trafficking by Mutation of Its PI-3 Linase Binding Sites

Discussion

Member takes

Implication

Key Points

Cite This Study

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